Tisagenlecleucel — Drug Monograph

Brand names: Kymriah

Drug class: Other

Mechanism of Action

Tisagenlecleucel is an autologous CD19-directed chimeric antigen receptor T-cell (CAR-T) therapy. Patient T cells are harvested via leukapheresis, then genetically engineered ex vivo to express a CAR containing an anti-CD19 scFv fused to 4-1BB and CD3ζ signaling domains. Upon reinfusion, the CAR-T cells recognize and bind CD19 on malignant B cells, triggering T-cell activation, proliferation, and cytolytic killing. The 4-1BB costimulatory domain promotes sustained in vivo persistence and memory formation. Lymphodepletion with fludarabine and cyclophosphamide prior to infusion enhances…

FDA Indications

Common Side Effects

Clinical Pearl

Approximately 1-month manufacturing lead time requires early referral planning. Bridging therapy may be required for aggressive disease. B-cell aplasia is a surrogate for ongoing CAR-T activity but necessitates indefinite IVIG replacement. Tocilizumab should be available at bedside before infusion begins.

Related Therapies & Mechanisms