Treosulfan — Drug Monograph

Brand names: Grafapex

Drug class: Alkylating Agent

Mechanism of Action

Bifunctional DNA-alkylating (busulfan-related) prodrug. Under physiologic conditions treosulfan converts non-enzymatically to reactive epoxide intermediates that alkylate and cross-link DNA, producing potent myeloablative and immunosuppressive activity that clears host hematopoiesis to enable donor engraftment. Its predictable, pH- and temperature-dependent (non-enzymatic) activation gives more consistent exposure than busulfan and does not require therapeutic drug monitoring.

FDA Indications

Common Side Effects

Clinical Pearl

Treosulfan (with fludarabine) is a myeloablative conditioning backbone that improved overall survival versus busulfan-fludarabine in older or comorbid AML and MDS patients (MC-FludT.14/L). Its non-enzymatic, exposure-predictable activation means - unlike IV busulfan - no pharmacokinetic therapeutic drug monitoring or PK-guided dose adjustment is needed, simplifying conditioning in a higher-risk transplant population.

Related Therapies & Mechanisms